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LAST MONTHS ISSUE | HOME | JANUARY ISSUE PAGE 1| JANUARY ISSUE PAGE 3

GREEN WATER: The Culture of Marine Phytoplankton
WINTER LIVE ROCK MAKING UPDATE
SOME LESSONS IN CURING LACE ROCK
THE GEMINI WATER PUMP
Colorful Live Rock Organisms, Sponges and Tunicates

Reef Aquarium Farming News
Online Newsletter for Reef Aquarium Propagation Research

ISSUE # 13 page 2 JANUARY 1998


Green Water: The Culture of Marine Phytoplankton

Rob Toonen
University of California, Davis

The first step necessary to consider breeding any animal is the establishment of a reliable food source for the young. In general, hobbyists have little trouble providing for the needs of the adults, but frequently the care of the offspring requires more specific foods, and are more difficult to provide. This is especially true of most marine species, which have a biphasic life (there are two distinct life stages: larval and adult). Generally among species with a larval and adult stage, the larvae live in different habitats or feed on different prey items than do the adults.

Approximately 80% of marine organisms (about 90,000 species), both vertebrate and invertebrate, have such a biphasic life-cycle and produce planktonic larvae which spend some variable amount of time (ranging from minutes to months) developing in the water column before settling and metamorphosing into the adult life-form (Thorson 1964). True to form, these larvae are planktonic (live as freely-floating particles in the ocean), unlike the majority of the species in their adult forms, and generally tend to feed on entirely different foods than do the adults. As if the care of young with specialized feeding requirements were not enough of a hurdle to the hopeful home breeder, the majority of these marine larvae are microscopic and require suitably tiny prey items to ingest.

However, for those truly adventuresome (or foolhardy) souls
who don't consider asexual propagation of their tank inhabitants
enough of a challenge, sexual propagation (involving the production
and fusion of gametes through either copulation or free-spawning)
of their reef inhabitants is the penultimate challenge.

Providing these suitably tiny particles is where the culture of phytoplankton (typically single-celled marine plants -- encompassing algae, diatoms, dinoflagellates, etc.) comes into play. Although this seems like an obvious an trivial thing, most people who have raised both phytoplankton and larvae consider the culture of marine invertebrate and fish larvae simple by comparison. If you plan to try breeding any of the critters in your aquarium, your first step should be to set up and successfully maintain a series of pure cultures of at least one, and hopefully several species of marine phytoplankton with which to feed any offspring produced. This sounds easy, but is generally much more difficult than it sounds. Many people have more trouble getting the algal cultures running well enough to start raising larvae than they do raising the larvae themselves.

First, let me start with some of the basics. There are two ways in which animals can reproduce: sexually and asexually. If the animals are asexual (no gamete production or fusion is involved), then a single individual can split itself into multiple copies (through various methods) and develop into a genetically identical colony to the original "parent" colony. In this case, there is obviously little special care required for propagating such animals at home -- if the parent colony can survive, then similarly good conditions and plenty of food usually suffice. Asexual reproduction broadly covers both the addition of units (such as cnidarian polyps or bryozoan zooids) to an existing colonial growth form, and the fission of individuals to produce two (or more) identical individuals that are not attached in any way (such as some cnidarians, echinoderms and polychaetes). The folks at GARF have dedicated a lot of time and energy to teaching hobbyists successful methods of asexual propagation of marine organisms. These web pages and newsletters provide a lot information regarding their techniques for successful asexual propagation of reef animals, and for most hobbyists, that is an admirable goal. However, for those truly adventuresome (or foolhardy) souls who don't consider asexual propagation of their tank inhabitants enough of a challenge, sexual propagation (involving the production and fusion of gametes through either copulation or free-spawning) of their reef inhabitants is the penultimate challenge. I give more detail on the breeding modes of marine invertebrates and larval culture in a series of articles for the Breeder's Registry periodical, The Journal of Maquaculture (Toonen, 1996a, 1997 a,b,c), and I discuss the biology and culture of rotifers in an article for Aquarium.Net (Toonen 1997d) -- but those subjects are not the focus of this article.

Instead, here I am going to concentrate on the most critical and often difficult step in the successful culture of marine invertebrate larvae: establishment of a successful and dependable phytoplankton culture. I assume that the average aquarist does not have the resources to purchase all their live food from a facility like Harbor Branch Oceanographic Institution (HBOI), so I will describe instead how to do it on your own at home. The first step to successfully culturing phytoplankton is obtaining both the nutrient supplement and the pure strain algal inoculant for your cultures. The supplement can come from a number of sources. First, you can buy sterile, premixed and measured aliquots of f/2 algal growth supplement from companies such as Carolina Biological Supply (1-800-334-5551), or Trans-Mississippi Biological Supply (1-800-544-5901). This is probably the easiest but also the most expensive way to feed your algal cultures.

Second you can buy bulk f/2 supplement from many aquaculture supply companies like Aquatic Ecosystems, Inc (1-800-422-3939), Fritz Aquaculture - now called Aquacenter (1-800-748-8921), Florida AquaFarms -- a division of HBOI's Aquaculture Facility -- (904) 567-8540, who provide a pre-mixed supplement which can be measured and added to culture vessels as needed. This second alternative is probably the best for people who want things as easy as possible, but still relatively inexpensive. The final method is the cheapest, but also the most work: you can mix your own nutrient supplement. Strathmann (1987) has a recipe based on Guillard's (1983) medium and the comments and adjustments of Bidwell and Spotte (1985), and Guillard (1975) is considered the authoritative source for all subsequent instructions and nutrient supplement recipes. The Botany Department at the University of Texas also sells media, and have an impressive list of the most up-to-date growth media recipes on their web site.

Alga-Grow Medium Recipe:

Filtered seawater 950 ml +
Deionized (Distilled or RO) water* 50 ml +
1 M KNO3 1 ml +
IMR Metals Solution (see below) 1 ml
Sterilize the solution (sterilization techniques are discussed below) and then add:
12 mM KH2PO4 5 ml +
Vitamin Solution (see below)* 1 ml +
Antibiotic Solution (60-150 ppm - see below) 1-2.5 ml

* If culturing diatoms, add 7.5 g of silica to 250 ml of DI water to make a stock solution, then add 50 ml of this solution rather than the 50 ml of DI called for above; if this forms a precipitate (ppt), cut it in half and try again until no ppt forms - the amount possible to add will be dependent on the hardness of your water. Also add 5 ml of "diatom booster" (see below) along with the vitamin stock after sterilization.



Wrap the container completely in tinfoil and either freeze or store at 4C. I usually split the medium between several containers of useful size (e.g., 250 ml) and freeze those I am not using, and keep the one I am using in the fridge.

IMR Metals Solution:

Stock 1: 48 mg ZnSO47H2O (or 30 mg of ZnSO4H2O) +

25 mg CuSO45H2O +

30 mg CoSO47H2O +

3.0 g H3BO3 in 1 liter of DI.

Stock 2: 25 mg Na2MoO42H2O in 1 liter of DI.
Stock 3: 5.0 g FeCl36H2O +2.0 g MnSO4 in 100 ml DI (ignore ppt and use clear liquid on top).
Stock 4: 50.0 g Na2EDTA2H2O in 1 liter of DI.

Again, all these stocks should be wrapped completely in tinfoil and stored in the fridge or frozen (it is not vital that these stocks be kept dark, but it does not hurt). To make up the IMR metals solution which is added to the medium, add 10 ml of each stock solutions 1,2 and 3 to 850 ml of DI water. Then add 100 ml of stock solution 4, and adjust the pH to 7.5 with dilute Nah. Finally, add enough DI to make the final volume 1.0 liters.

Vitamin Solution:

0.5 mg Cyanocobalamin (Vitamin B12) +
0.025 mg Thiamine +
0.25 mg Biotin in 250 ml of DI.

It is essential that this stock be kept dark and cold for long-term storage. Make sure the tinfoil wrap is complete on this stock, and store at 4C or, preferably, frozen.

Antibiotic Solution:

15.0 g Penicillin G +
15.0 g Streptomycin in 250 ml of DI.


Again, it is essential that this stock be kept dark and cold for long-term storage. This stock needs to be frozen in tinfoil if you intend to keep it for more than a month or so. I simply take my frozen stocks out of the freezer and place them in the fridge to thaw overnight before I mix new medium, and then immediately replace them in the freezer when I am done. The amount of antibiotics added to your cultures is both a matter of preference and need. Mike Hadfield's group at the University of Hawaii use 60 ppm of Pen/Strep, whereas Dan Morse's group at UC Santa Barbara use 150 ppm. I personally use 60 ppm if I use any antibiotics at all, but IF you find it necessary to add antibiotics, I would suggest that you start with 60 ppm (1 ml of stock per liter), and increase that up to 150 ppm (2.5 ml of stock per liter) as necessary.

I say IF you find it necessary, because I am personally opposed the superficial and ignorant use of antibiotics at home. With improper disposal - like simply dumping the stock down your sink or toilet - new bacteria are repeatedly exposed to the antibiotic, and eventually some individual that is resistant to that compound will be exposed to the antibiotics, have a huge selective advantage and rapidly spread. Chalk up another useless antibiotic to carelessness... If you are going to use antibiotics for treating your cultures, please do everyone (including yourself) a huge favor and boil your "waste" water before disposing of it.

Diatom Booster:

1.7 g Sodium Glutamate
0.2 g Sodium Glycerophosphate in 1 liter of DI.

Store in the dark and cold as suggested for the stock solutions above.

Once you have the nutrient supplement, you'll need an inoculant to start your cultures. Again, you have a number of options. First, you can simply the pure strain cultures to feed your larvae from a company such as HBOI. If you can afford to buy 20L flasks of culture from HBOI, you probably don't need to be reading this article, so the fact that you are suggests that you need to culture your own. The best place to obtain cultures is undoubtedly the Culture Collection of Algae at The University of Texas at Austin if you're looking for a specific alga, and you know the species, but there are other suppliers like Carolina Biological Supply, or Trans-Mississippi Biological Supply. Again this first option is the easiest and probably most expensive method of obtaining cultures, but in this case also by far the most reliable. Second, you can try to get inoculant from some local source (check with a University if there is one in your area, or try to contact other breeders in your area to "borrow" some of their culture). Third, you can try to isolate algal cells from natural seawater, or even possibly from well established reef tanks. This third option will again be the cheapest, but also the most work and by far the least reliable (you could as easily isolate a toxic dinoflagellate as a useful diatom). I would only recommend home isolation of algae if cost prohibits the purchase of known algal stocks. The easiest way to do this - if you have a good microscope handy - is to examine a droplet of water under a microscope and remove a single cell of an appropriate diatom or alga for culture (don't get greedy! One cell is really all you need, but set up several replicate cultures in case of contamination).

The species recommended and used by the folks at HBOI aquaculture facility include: Isochrysis galbana, Chaetoceros gracilis and Thalasiossira weissflogii. There are many other species of algae commonly used for larval culture (as anyone who has visited the Utex site will quickly discover), but I will only discuss these three here.

1) Isochrysis galbana -- gold-brown alga on the order of 5-8 micrometers which contain large lipid stores (this species produces many highly unsaturated fatty acids). An excellent food for most small planktotrophic species, especially in mixture with Chaetoceros or Thalassiosira. Especially recommended for mollusc larvae, copepods and rotifers.

2) Chaetoceros gracilis -- a relatively small-diameter chain diatom, averaging about 20% lipid. A good supplemental food for most late stage or crustacean larvae, and an excellent food for adult bivalves.

3) Thalassiosira weissflogii (=fluviatilis) -- large diatom, averaging about 20 or so micrometers in length. An excellent food for juvenile and adult bivalves, and a good food for echinoderms and crustaceans.

In order to be at all successful in culturing "pure" strain algal cultures, you will first need to sterilize the seawater in which algae are to be introduced. The best way to do this is, of course to autoclave it, but this is not always easy or desirable for the hobbyist at home. That leaves a couple of options. The first, if you have the equipment available or money and desire to buy it, is to Millepore filter your seawater to one micrometer (there aren't any cheap alternative filters I know of to get 1 micrometer filtered seawater - you have to bite the bullet and go for the Millepore stuff). This could be made cheaper (actually only by making each filter last longer before clogging and becoming useless) if you drip the water from your tank through a coffee filter (I HOPE coffee filters are not exposed to formalin in processing the way paper towels are!! I think they are oxygen whitened). The second method is much cheaper and more likely to be used at home.

Microwaves have been found to kill most everything but fungal spores, so if you live in a relatively dry part of the country, and fungal spores are not a source of significant contamination, you need only microwave your culture medium. Guillard found that the medium need not even boil for the microwaves to kill most things in seawater. Generally if you place the medium in the microwave in the flask in which you intend to culture the algae for about 2 minutes on high, you'll have pretty sterile culture medium. If, however, you happen to live somewhere like Florida or the Carolinas, where fungal spores will definitely be a major problem, you'll need to get a special microwave pressure cooker. I have heard of them, and know people who use them, but have never tried it myself. The major concern for a pressure cooker is that you add some fresh water to the inside of the cooker (outside the flask) to prevent your algal growth medium from boiling over and making not only a mess, but wasting the medium.

BRINE FLASK

The final method of sterilization is pasteurization. You can pasteurize filtered seawater by heating it to 60-65C for at least 30 minutes or to 85-90C for at least 2 minutes. Once heated, you should let the medium cool and sit at room temperature for 24 hours before a second pasteurization to kill any germinated spores (Jebram 1977). After the second round of heating, the medium should be moved directly to a fridge for cooling and equilibration, and allowed to sit with occasional gentle agitation for at least 24-48 hours. Pasteurized seawater should be stored at 4C until used (but be sure to always warm the water before using it). Of course, a combination of as many of these techniques as is available to you (e.g., pasteurization in a microwave pressure cooker) will increase your chances of obtaining a relatively sterile medium.

After sterilizing the medium (you should always sterilize the medium within the flask in which you plan to culture your algae to minimize the opportunity for contamination), you must aerate it for a while prior to adding the algal inoculant to remove excess carbon dioxide from the medium. Once the medium is sterilized (or nearly so), make sure that the stopper stays in the beaker, and that you never let anything come into contact with the medium or the edge of the stopper to go into the flask (that ESPECIALLY includes your hands - never touch the flask or stopper on the "inside" parts once sterilized). You can open the flask to add algal inoculant water and/or nutrient supplement, but make sure that you only lift the stopper enough to get whatever you're trying to add into the flask, and keep it directly over the culture flask to prevent anything settling into the flask while you're doing that. While the stopper is being held above the opening, make sure that neither the stopper nor the glass tubes touch anything, and replace the stopper immediately after adding new medium. If you plan to get into this seriously, I would suggest that you find a few glass 1 or 2L Erlenmeyer or Fernbach flasks (large flat-bottom, narrow-necked flasks) and get a good quality rubber stopper that tightly fits each flask. There are as probably as many methods of setting up your cultures as there are types of algae cultured, so I won't go into excruciating detail on the technique here, but will instead show you with how I maintain my algal cultures.

You basically want an airline tube to go into the culture and one to let air out of the culture (see the photograph of our setup here). Beyond that, you can add extras, like an outlet tube to "pour" culture from (see Toonen, 1996 for details), but although that is nice to have, it is not necessary to keep algae successfully. The main thing is to keep the algal cultures sterile -- everything else (feeding, lighting, water quality, etc.) is relatively forgiving by comparison, and much easier for the typical aquarist to control.

ALGAL CULTURES

Once you have the thing set up, and are ready to add the algae, you can set the flasks on a shelf in front of a window that gets lots of sunlight, or set up a light specifically for it - which ever works best for your needs. Both GE and Phillips make cheap full-spectrum lights that are good alternatives to spending a mint on petshop aquarium bulbs (e.g., GE Chroma 50, Phillips color tone 50). I'd use at least 80 W of light directly above (or better yet around/beside -- you'll get better growth with a single bulb beside the cultures than two directly above it) the flasks if you want decent growth and final culture densities. I use 160 W of full spectrum light from the side (lights are mounted into the door of a light box which is sealed and with reflective Mylar all around the inside), and I don't start to feed the cultures to my larvae until they reach about 10,000,000 cells per ml (which takes about a week to ten days. Home use probably doesn't require anything so drastic, unless you plan to run a major breeding facility. Your cultures are probably fine if they look dark green or brown. The recommendation Guillard makes for lighting is to provide about 4000-6000 lux for about 14 hours per day. This is about the output I get at 10-14" away from the four 48" 6500K full spectrum lights (I have to use the old bulbs retired from my reef tank for something) in my light box door, just to make this archaic lux measurement into something tangible for most people (you'll get more photosynthetically active light from the cheap GE/Phillips bulbs mentioned above or even cool whites than from specialty aquarium bulbs, but I don't notice any difference in growth regardless of the brands/types of bulbs I tried).

Once you have a reliable supply of marine phytoplankton cultured, you're ready for the next step -- breeding tank inhabitants that produce feeding larvae, but that is another story for another time.



Literature Cited:



Bidwell, J.P. & S. Spotte. 1985. Artificial seawaters: Formulas and methods. Jones and Bartlett Publ., Inc. Boston, Ms. 349pp.

Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates. In: Culture of Marine Invertebrate Animals. W.L. Smith and M.H. Chanley (eds.), Plenum Publishing Corp., New York, NY. pp. 29-60.

Guillard, R.R.L. 1983. Culture of Phytoplankton for feeding marine invertebrates. In: Culture of Marine Invertebrates: Selected Readings. C.J. Berg, Jr. (ed.), Hutchinson Ross Publ. Co., Stroudsburg, Pa. pp. 108-132.

Jebram, D. 1977. Experimental techniques and culture methods. In: Biology of Bryozoans. R.M. Woollacott and R.L. Zimmer (eds.), Academic Press, New York, NY. pp. 273-306.

Levin, L.A. & T.S. Bridges. 1995. Pattern and diversity in reproduction and development. In: Ecology of Marine Invertebrate Larvae. L. McEdward (ed.), CRC Press, Boca Raton, FL. pp. 1-48.

Peirson, W.M. 1983. Utilization of eight algal species by the Bay Scallop Agropecten irradians concentricus (Say). J. Exp. Mar. Biol. Ecol. 68:1-11.

Strathmann, M.E. 1987. Reproduction and Development of Marine Invertebrates of the Northern Pacific Coast: Data and methods for the study of eggs, embryos, and larvae. University of Washington Press, Seattle, WA. 670pp.

Thorson, G. 1964. Light as an ecological factor in the dispersal and settlement of larvae of marine bottom invertebrates. Ophelia 1:167-208.

Toonen, RJ. 1996. Invertebrate Culture, Part 1. The Journal of MaquaCulture 4(4):6-26.

Toonen, RJ. 1997a. Invertebrate Culture, Part 2. The Journal of MaquaCulture 5(1):4-13.

Toonen, RJ. 1997b. Invertebrate Culture, Part 3. The Journal of MaquaCulture 5(2):31-37.

Toonen, RJ. 1997c. Invertebrate Culture, Part 1. The Journal of MaquaCulture 5(3):41-51.

Toonen, RJ. 1997d. Reefkeeper's Guide to Invertebrate Zoology: Part 5, Phylum Rotifera. Aquarium.Net, March '97:7 pp.


We are happy to announce that Tim L. Weidauer has purchased the famous GREEN WATER AND ROTIFER SOURCE Wasatch Aquaculture in Salt Lake City. His green water may be the most hardy culture in the hobby. We have frozen and thawed these cultures over 30 times during the past month, and the green water is still thriving. We have just thawed his Rotifer cultures and they are now producing strong populations. These strains allow us to freeze 1/2 of the orders we receive every two weeks. Please visit Tim's web sites.

Tim L. Weidauer OWNER- Wasatch Aquaculture -http://www.rotifer.com - "Live Plankton!"

President - Wasatch Marine Aquarium Society - Salt Lake, Utah


WINTER LIVE ROCK MAKING UPDATE
FROM UPSTATE NEW YORK

Happy New Year to all, and may your year bring you all that you desire in it. This is the year that we bring knowledge of the Reefs to all that we can. As Sally Jo says,"think globally, act locally". That means to increase our activities in captive marine reef propagation, and, making our own rocks for our corals to live on.

jakerock

Winter is seriously here in now in upstate New York, and our rock making has moved from my associate Joe Woods' carport to his basement, and we are making our rocks while ducking the low overhead! Work bump, ouch, work, bump ouch! 'All for the cause'! Cause no place else to do it now! But, it goes along, and we have progress, and learn more as we go along.

An incident prompted me to send in this update on what we are doing.

I asked her what she had thought of the rock
I had brought up and she replies,
" oh, didn't you see it, I put it here".

It was right next to the rock that
I had been looking at for 15 minutes
and I did not even recognize my own rock!!!!!

jakerock

The later part of Dec I was visiting Carribean Forest marine shop in Rochester and browsing through the shop, taking my time and enjoying their inventory of marine fish and corals. In fact, Milly the owner operator now has a sale tank of totally cultured corals for sale! She also has a huge selection of imported ones. Anyhow, there were a number of customers in the store so I was browsing around.

jakerock

They had moved the big tank with all of the sale Fiji live rock and I noted a piece that I had made of ours and brought up to show them was no longer in it, no big deal, just noticed it wasnt in where Dan had put it when I dropped it off. I spent awhile studying one piece of flat 'plate/ledge like' rock that was a lot like some Joe and I have been making, for building reefs, we make some of them flat, and then put caves or other raised rocks on and under the 'plate rocks'. This looked nice and had a lot of nice natural coral growth on it and I was debating buying it for an example but finally decided that it was close enough to what we were making that we were o k as we are so I kept looking around but stopped back a number of times to admire and study that flat piece.

So, in a way, this was the first real 'acid test' of our rocks, putting
them in a store display tank with sale stuff and not being able to tell
them from the natural product! I felt quite good about this as I drove
off, and, now know that we are making a good, natural looking saleable
product in our rocks!

And, they are good enough, that even the maker wont recognize them!!!

A while later when things had slowed up a bit and I was sipping cofee with Milly and another couple of her old customers I asked her what she had thought of the rock I had brought up (she wasnt there when I dropped it off and her husband Dan took it), and she replies, " oh, didnt you see it, I put it here'", while pointing to the tank where I had been studying the 'ledge rock'. It was right next to the rock that I had been looking at for 15 minutes and I didnt even recognize my own rock!!!!!

jakerock

It was totally coralline covered, and she had placed it up in the same layer as the ledge and I looked right at it and never recognized it! The couple of 'old customers' at first didnt believe it was manmade, but we finally convinced them by having them look UNDER the hollow center and seeing the arragocrete undersurface. This was one of our earlier arragocrete/oyster shell mix rocks, about 8" in diameter, made of a stiff mix built up over a core pile of damp arragonite gravel with a stiff arrogocrete mix with 1/5th ziti in the mix. The ziti sloughs out and the residue hosed off with a kitchen pressure hose and then we cure them for a month in freshwater and a couple weeks in salt water before use. Building it up over the damp pile of oyster shell gives it a hollow interior when lifted off the pile. We use the slow cure in water just to be totally safe of no ph shifts, better safe then sorry, when they are algae encoated then they are safe to use.

We are also using this technique on the 'Cave Rocks'
that LeRoy wrote about and this makes fabulous looking reef rocks.
There are any number of variations that can
be done on this.

Your imagination is the only limit,
just stick with these basic guidelines.

So, in a way, this was the first real 'acid test' of our rocks, putting them in a store display tank with sale stuff and not being able to tell them from the natural product! I felt quite good about this as I drove off, and, now know that we are making a good, natural looking saleable product in our rocks!

And, they are good enough, that even the maker wont recognize them!!!!!!!

So, what are we doing? Refer back to the previous Garf newsletters on the overal methods. We use a combo of #3 Portland Cement, but 1-11 will also work if you just cant get the #3, our local lumber dealer special orders it for us now and we like the local supply that this gives us. At first we used all arragocrete gravel, but, now intermix it with oyster shell from a feed dealer, and use the oyster shell now for making damp mounds to make the raised hollow core rocks. I make those by trowelling a stiff mix from the base of the oyster shell up over it and leaving 1-2'' holes on each side as I build it up so that when cured a clown or damsel sized fish can swim right in one side and out the other.

There is a lot of good info for you now in rockmaking.
Each person has their own touch
now it just remains for you to add your own to these!

Give it a try, and let us know how you are
doing, send photos!

Others we also make with solid sides and a 'cave opening' on one side. By using a stiff mixture we get a very rough textured surface that has a very natural appearance when cured, and also has a multitude of crevices for microfauna to colonize. The ziti in the mix also gives a number of hollow tube like holes or long holes if on its side in the surface that greatly enhances its apperance and helps give it a more porus look, and more room for the microfauna. The oyster shell also sticks to the underside and gives it a more natural look.

For an 'extra touch' I also take a couple sizes of 'shell macaroni', dry, and impress them into the surface of the rocks, maybe 3-4 per rock, when removed you have a nice 'fossil-like' shell imprint in the rock. All in all this makes a very attractive and useable rock in the reef tank. Once it is encrusted with corraline it is very difficult to detect from a naturally made rock, as I found out to Millys' delight. One of the few times that I have really enjoyed the laugh being on me.

jakerock

We are also using this technique on the 'Cave Rocks' that Leroy wrote about and this makes fabulous looking reef rocks. We have made a couple of 'giant ones', and even 'double' ones by just making a hard bridge between two caves as they were poured. There are any number of variations that can be done on this. Your imagination is the only limit, or just stick with these basic guidelines. We also make flat 'plates' for other rocks to stack on and to build ledges with. We try to make the base rocks sitting on the tanks gravel to have hollow cores so as to minimize rock sitting on the surface of the gravel and preventing water contact with the gravel surface.

This is the Internatuonal Year of The Ocean
and what better way to teach your kids, and others'
about the reef then by teaching them how to
build their own?

Our present variation on 'plugs' for mounting coral cuttings is to put about a tablespoon of stiff ziti mix into an ice cube tray, and cure it and use it. This gives us a piece of rock about 1-2', irregular in shape and rough textured and porus to mount coral cuttings on, and these easily superglue onto our cured rocks and look natural when mounted on them. This is probably our method from now on for' cutting' rocks.

There are several other variations that you can do, using thin spaghetti will give very tiny long holes in the mix but its slower to cure out and could take a couple of months to 'naturally' soak out. I like using pasta as it is easiest to remove and leaves good sized holes in the mix when removed that look natural. Small pasta is tempting, but slow to dissolve or remove.Maybe with the help of crayfish in the freshwater cure? Worth trying. Now could also be the time to look for large amounts of Christmas hard candy to mix into the mix, when dissolved these could leave interesting holes in the mix. I try to build my rocks with about an average 1" thickness on the walls, this seems strong enough to withstand handling, and looks good.

By March we plan to have several 150 gallon plastic horse tanks curing rocks to use. One of these is simply going to have the water drained then salt water put in and begin the culture of corraline right on my back deck, I am also going to seed the rock with copepods and rotifers right there, along with the bacteria from some selected live rocks(my make) with no bristleworms or other baddies on them. By summer or fall we should have some gorgeous liverocks ready to mount cuttings on, if, we havent already used them all up!

There is a lot of good info for you now in rockmaking, from Leroy and Karen Holts and Tom's articles and each has its own personal touch, now it just remains for you to add your own to these! Give it a try, and let us know how you are doing, send photos! The new kids reef site is a great place to get your own younguns involved, and Karens ' article in the Dec newsletter is a classic example of how to go about it involving your children in this fascinating pursuit. This is the Internatuonal Year of The Ocean and what better way to teach your kids, and others' about the reef then by teaching them how to build their own?

Sally Jo says, "think globally, act locally". For those of a religious persuasion, this is in keeping with the First Commandment in the Bible, in Genesis it says, roughly translated from the Hebrew, "take possesion of the Earth, and REPLENISH it"! This is a great way to start. Let all of us know how we can help you.

enjoy!



LEARNING EXPERIENCES - SOME LESSONS IN CURING LACE ROCK

LeRoy has asked me to write about some of my learning experiences with cement based reef aquarium rock. I make both Aragocrete and crushed oyster shell rock. I am a very new aquarist and I have only been making "rock" since late fall. The learning experiences worth noting are those of impatience, inexperience and assumption.

karen rock

In the beginning I made several batches of rock and placed them in a kids swimming pool in the backyard and changed the water everyday for several weeks and then ASSUMED that the rocks were cured sufficiently to add to my reef tank. I must have spent two hours getting the arches, tunnels and shelves in the right place. I got them in the tank and within an hour there was something very wrong with the inhabitants. They were not swimming or eating , they were gone to the sleeping/hiding places. I quickly pulled the rock out and changed the water as quickly as I could. 90 gallons is a lot of water to change quickly.

THESE PICTURES SHOW HOW THE YOUNG ONES LOVE TO HELP MAKE REEF ROCK

karen rock   karen rock

The next case of tank crash was about two months later. Again, in my impatience and inexperience I assumed that the rock that I had soaked in vinegar for several days and then changed fresh water on for about two weeks was sufficiently cured. Again I placed about 150 pounds of rock in the tank and within the hour I knew that I had done it once again. I emptied the tank quickly and changed the water. The next day the inhabitants still looked distressed, so I changed the water again. All 95% of a 90 gallon tank. On that day I lost some beautiful fish , a lot of sleep and was physically stiff and sore for quite a while.

karen rock

Now, in my old age I have learned that Aragocrete and crushed oyster shell rock needs to be completely cured before adding it to a reef tank. I have purchased a pH testing kit and USE it before placing rock in a tank. For me and my higher percentage cement rock perhaps the low slow cure in fresh water is the best.

karen rock

(The Aragocrete formula is Aragonite to cement at about 5 or 6 to 1. The oyster shell rock that I have been making is about 3 to 4 parts crushed oyster shell to 1 part Riverside white cement.)

Karen, the Rocker

AT GARF WE CURE OUR ARAGOCRETETMIN FRESH WATER FOR SEVERAL WEEKS AND THEN WE SOAK IT IN HOT WHITE VINEGAR. WE THEN RINSE THE ROCKS BEFORE PUTTING THEM IN THE REEFS. We have NEVER had any problems with pH in any reef aquarium.
LeRoy


Reef Aquarium Aquaculture Products Testing Laboratory

Reef Aquarium Aquaculture Products Testing Laboratory Consumer Reports


THE GEMINI WATER PUMP

Approximately eight years ago, a water pump was introduced to the market that was made in Taiwan under the auspices of Aquarium Products of Glen Burnie, Maryland that was different.

Its original design was to be used in conjunction with a versatile filter that sat on the top of aquariums, yet allowing a hood or cover and many fluorescent tubes with end-caps. This water pump could also be used by itself because a corner holder that fastened easily to the aquarium was included.

A big advantage is that this pump has the motor on top of the aquarium - not in the aquarium; so that the heat is not a detriment. Yet, it is silent; easy to clean without having to unscrew anything if necessary; it will not be injured by pulsing on and off and it pumps 960 gallons an hour enough to move the surface of a 150 gallon aquarium like a wave. It is economical to operate and our experimental ones have been running as described above in marine aquariums since introduced. The Gemini compares favorably, if not superior, to the Tunze water pumps but is considerably less expensive. During these past eight years, the Gemini Water Pump has developed a following with hobbyists using them for revolving and circulating. Aquarium Products is a reputable company and, like all their products, stands behind the high quality of this non-submersible, unique water pump.

Merrill Cohen the owner of Aquarium Products

We have been using these pumps for the last 3 years and they have worked very well. This is one of the only pumps that can continue to operate when it is on a constant on off mode such as a wave maker. The Gemini pump moves a very large volume of water. If you need a pump that will not increase the temperature of your reef and will survive the hard use of a wave maker then you will be very happy with this product.


Colorful Live Rock Organisms, Sponges and Tunicates

By Tom Frakes
Anclote Aquaculture, Inc.

The Eastern Gulf of Mexico off Tarpon Springs is a rich area particularly in regards to encrusting organisms. This area is well known for the Greek sponge industry that developed earlier this century. Now it is the home of several live rock farms. In addition to several species of hard corals that grow on the rock are a host of colorful sponges and tunicates.

tom's rock

These two groups are very similar in appearance to the naked eye, yet are quite different. Sponges are very simple organisms while at the other extreme, the tunicates are highly developed animals including a nervous system. Identification is often quite difficult for both of these groups as growth patterns are strongly affected by environmental conditions so shape and color can be quite variable. Both groups produce thin encrusting as well as lumpy shaped masses. In general the sponges are more colorful. Currently, we are harvesting rock with a range of colorful sponges.

tom's rock

Orange sponges come in a number of forms. The Orange ball sponge (Cinachyra sp.) is a distinct ball shape while the encrusting orange sponge (Mycale laevis) and the orange lumpy sponge (Ulosa hispida) can have many shapes. There are also yellow boring sponges (Siphodictyon sp. and Clioa sp.) that can appear as yellow dots or an encrusting mass. Red encrusting sponge masses (Diplastrella sp.) offer another attractive type. Encrusting tunicates can also be found that include brown (Botrylis sp.), red, blue-grey (Tridemnum sp.), white (Didemnum sp.), etc. The most dramatic are the blue sponges. We have at least two varieties. One has a spiky texture with miniature tubes (Dysidea sp.) and another that appears to be a blue variety of bread crumb sponge.

tom's rock

These blue sponges are thought to be the food of the colorful Greek goddess nudibranch (Hypselodpris edenticulata). These beautiful creatures only feed on this sponge so some wonder if they should be kept in reef tanks. On the other hand they may only live a few months in the wild. We just started to see them again this month after an absence on the reef since last spring. It has been suggested that their natural seasonal life cycle may be quite short and they may last as long in the aquarium as on the reefs.

tom's rock

Sponges may be a useful addition to reef tanks as they are filter feeders specializing in very small particles such as bacteria and smaller. Because they feed on such small particles, from several microns to less than a micron, they may not be competing with other filter feeders that eat zooplankton or algae. In theory they could reduce bacterial levels in the water taking out things that pass through the finest mechanical filters. One problem with sponges is that they don't ship well dry. In fact some are reported to do poorly if taken from the water even briefly. For best results rock with sponges are shipped wet or at least partially covered with water. For best success shorter shipping time are best, 24 hours or less.

FREE INFORMATION ABOUT PURCHASING

TOM FRAKES AQUACULTURED LIVE ROCK

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CORALLINE ALGAE PROPAGATION Learn to grow coralline algae on your reef aquarium live rocks
COMBINATION ROCK PROPAGATION Learn to grow combination reef aquarium live rocks
COMBINATION ROCK BASE ROCK SELECTION Learn about base rock for combination reef aquarium live rocks
COMBINATION ROCK SPECIES SELECTION Learn about selecting species for combination reef aquarium live rocks

LOW COST BULLET PROOF REEF AQUARIUM Learn to start an inexpensive reef aquarium
55 GALLON INSTA REEF Visit Rache's 12 week old Bullet Proof Reef Aquarium
MORE PICTURES OF THIS REEF AQUARIUM


Geothermal Aquaculture Research Foundation,Inc. online tour Learn why we call it Geothermal
GARF RESEARCH HOME PAGE Learn about the other research we are doing
Super glue research page We teach you the Super Glue method of invertebrate propagation
Super Glue evaluation page Our researchers rate many brands of super glue
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Natural Algae Control Reef Janitor Order Page - red leg hermits, snails, grunge.

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Research page for Xenia and related soft coral propagation Learn to propagate xenia. Please enter any data you have about these corals.
Soft Coral Propagation Page Pictures and details of soft coral propagation
Stony Coral Propagation Page Pictures and details of small polyp stony corals
Mushroom Anemone Propagation Page Pictures and details of mushroom propagation
Zoanthid and palythoa Anemone Propagation Page Pictures and details of Sea mat propagation
Learn to construct a 140 gallon plywood and epoxy reef tank

Image Page for Zoanthids and Palythoa

WETLANDS SAVE THE WORLDS REEFS FROM ALGAE DESTRUCTION
LEARN ABOUT OUR WETLAND RESEARCH

Boise Wetland pond tour 97 Pictures and details of wildlife pond tour




Email: leroy@garf.org
LAST MONTHS ISSUE | HOME | JANUARY ISSUE PAGE 1| JANUARY ISSUE PAGE 3

GREEN WATER: The Culture of Marine Phytoplankton
WINTER LIVE ROCK MAKING UPDATE
SOME LESSONS IN CURING LACE ROCK
THE GEMINI WATER PUMP
Colorful Live Rock Organisms, Sponges and Tunicates


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